More precision for gene scissors
CRISPR-Cas9 has revolutionized molecular biology. The enzymatic system works like scissors that can cut DNA at a defined location to deactivate genes or insert new gene sequences. To make sure that CRISPR-Cas9 finds the right place to cut, it is combined with a target-specific so-called guide RNA. However, even this guide RNA makes a certain percentage of mistakes in finding the target.